Part:BBa_K243003:Design
Digoxigenin binding protein (DigA)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with Biobrick pre-and suffix for fusion proteins according to the RFC 25.
Commented GenBank file
Source
Planned and designed by Team Freiburg Bioware09 and synthesized by purimex.
References
Holtke HJ, Ankenbauer W, Muhlegger K, Rein R, Sagner G, Seibl R, Walter T. (1995)
The digoxigenin (DIG) system for non-radioactive labelling and detection of nucleic
acids--an overview. Cell Mol Biol. 41(7):883-905.
Roche Applied Science (2002) DIG Application Manual for Nonradioactive In Situ
Hybridization (3rdedition)
Moter A, Göbel UB (2000) Fluorescence in situ hybridisation (FISH) for direct visualization of microorganism, J Microbiol Meth. 41: 85-112
http://www.thermo.com/eThermo/CMA/PDFs/Various/File_29495.pdf (image)